[2] The original plan for the competition involved dividing the 24 teams from the top two tiers of English rugby into four pools of six.

The number of teams dropped to 22 following the suspension of Wasps and London Irish.

The teams were seeded based on league standings at the end of the 2022–23 season.

[15] [17] [16] [19] [20] The semi-finals were confirmed on 17 October after points from the two cancelled Jersey Reds fixtures were allocated.

Player of the Match: Cam Jordan (Gloucester) Assistant referees: Harry Walbaum John Meredith[22] Television match official: Dan Jones[22] Notes: [15][17][16][19][20][21][23] [15][17][16][19][20][21][23] [15][17][16][19][20][21][23] One game from each round will be broadcast on TNT Sports as well as both the semifinals and the final.