[5] Finnish driver Emma Kimiläinen dominated the opening session of the weekend, setting the first representative time and maintaining the top spot throughout.
The only driver in the field to have raced at Assen previously, having done so in 2008, would finish nearly three tenths clear of Friday gun Jessica Hawkins and Norisring retirement Sarah Moore.
Drivers would start pushing track limits, with Moore bouncing across the same kerb that caught out Hawkey whilst Fabienne Wohlwend would damage her front wing on another.
A closing minutes dash for times would ensue, however before drivers could complete their laps Piria would suffer from an engine failure out on the circuit and bring a premature end to the session.
Further back, a bottleneck at the first corner would see Tasmin Pepper lose the rear end on the wet kerb from earlier rain and spin, with an unsighted Miki Koyama crashing into her.
Holbrook would take a trip through the gravel at the final chicane at the back of the field, whilst Sabré Cook would receive a drive-though penalty for starting out of position.
With ten minutes remaining, Kimiläinen would force Powell into an error at the first corner – the Briton running wide and ceding the lead to the Finn.
Caitlin Wood and Gosia Rdest would achieve season-best results behind with fifth and sixth, while Jessica Hawkins would score her first points of the season in seventh.
Megan Gilkes would therefore start the race from pole position, ahead of reserve driver Sarah Bovy and Shea Holbrook.