[2] FC Cincinnati's offseason transfers and preseason training were executed under head coach Ron Jans.

However, Jans resigned on February 17, amidst an investigation into his alleged use of a racial slur.

The tournament was played within a bio-secure bubble near Orlando, Florida, and was followed by the resumption of the regular season with a modified schedule to reduce travel.

[5] FC Cincinnati played all of their home matches behind closed doors with no public spectators in attendance.

[6] Win Draw Loss Due to their final position in the 2019 MLS standings, FC Cincinnati would enter the competition in the Third Round, which was scheduled for April 19–21.