The competition was contested by the thirty-two teams that took part in the 2019–20 season, which was suspended and subsequently abandoned due to the COVID-19 pandemic.
In both stages, the teams were placed in groups according to the ones they were in for the previous season.
The winners of that knockout tournament earned the second promotion berth to the Liga Profesional.
Team 15 and so on, playing a single match on neutral ground.
[1] The semi-finals were contested by the three winners from the previous stage, as well as the First Promotion final losers Estudiantes (RC).