The 2021 Big 12 Conference softball tournament was held at ASA Hall of Fame Stadium in Oklahoma City, Oklahoma on May 14 and May 15, 2021.

The Oklahoma Sooners came into the tournament with the best record from conference play at 16–1.

The winners of each pool advanced to the championship game.

The second- and third-place teams in each pool played in classification games for third and fifth place, respectively.

2 Oklahoma State The following players were named to the all-tournament team:[4]