Babiš ran again as leader of ANO, and the main opposition parliamentary parties formed two electoral alliances, SPOLU and Pirates and Mayors (abbreviated to PirStan or PaS).

[7] The opposition parties won a majority in the Chamber of Deputies and agreed to form a coalition government with SPOLU leader Petr Fiala as the new prime minister.

[8][9][10] Traditional left-wing parties ČSSD and KSČM failed to reach the 5% threshold to win any seats in the Chamber of Deputies for the first time since the dissolution of Czechoslovakia in 1993.

[57] Czech Republic invited experts from Organization for Security and Co-operation in Europe (OSCE) and Office for Democratic Institutions and Human Rights (ODIHR) to observe election.

[61] Simultaneously with the election, there were local referendums held in 20 cities and towns: Blšany u Loun, Blučina, Bzenec, Dačice, Drnholec, Jáchymov, Jezeřany-Maršovice, Katovice, Losiná, Pardubice, Podolí, Příkosice, Řevnice, Slavonice, Sušice, Ševětín, Temelín, Třemošnice, Velký Beranov, Zduchovice and Stará Bělá (part of Ostrava).

[84] The alliance's campaign was launched at a press conference on 16 June 2021, where Sehnal introduced APB's priorities, including support for Czech businesses and lower taxes.

[98][99] On 29 September 2021 Hungarian prime minister Viktor Orbán visited the Czech Republic and formally endorsed ANO at a rally in Ústí nad Labem.

Chairman Radim Špaček described the program goals, to reform the Czech state from a republic to a parliamentary monarchy, to restore the historical lands of Bohemia, Moravia and Silesia, and revise the legal order.

Deputy Chairman Petr Krátký cited a public opinion polling indicating that almost 10% of Czech citizens, especially younger voters, are in favor of a monarchy.

[107] Bartoš said on 11 January that the alliance's priorities during the campaign would include reform of the debt collection system, digitalisation, environmental issues, and education.

Bartoš and Rakušan promised to regulate debt collection, raise taxes on commercial buildings, and begin preparations to adopt the euro.

[111] At a press conference on 24 June 2021, Pirates and STAN launched an anti-corruption campaign, publishing a list of the 10 biggest corruption cases since 1989, primarily involving ODS.

[112] In July 2021, the alliance released a poster featuring Jakub Michálek, its candidate for Minister of Justice, trying to catch a man in a suit with a lasso, accompanied with anti-corruption slogans.

ČSSD also expressed their intention to implement progressive taxation and a lower tax on groceries, rejected privatisation of health care, and promised more achievable housing.

SPD used an election truck called "espéďák" to travel around the country, and attempted to attract potential voters to meetings by holding fairs with cheap food.

Led by Petr Fiala, the alliance launched its campaign on 9 December 2020, promising to reform tax, the social and pension system, and healthcare.

Its proposals included a minimum pension, a simplification of social benefits, support for education, and better use of EU funds as well as a focus on climate change.

Spolu also screened the film Women on the Run at its campaign meetings, and featured stand-up comedians including Petr Čtvrtníček in electoral adverts.

"[182] Million Moments for Democracy led a campaign against Andrej Babiš and ANO, attempting to persuade voters to support the opposition alliances Spolu or Pirates and Mayors.

Members of the movement talked to people in the streets and gave them leaflets in order to change their minds vote for one of major opposition alliances.

[262] Political analyst Jiří Pehe of the New York University in Prague hailed the results as a "triumph for liberal democracy" and said they "signalled the end of the post-Communist era".

He argued that in the last week running up to the election it had become clear that Petr Fiala was an equal opponent to Prime Minister Andrej Babiš, and competition between the two men led voters of smaller parties to switch to Spolu or ANO, leading to disappointing results for Pirates and Mayors and SPD.

[271] It was reported on 22 October 2021 that the Pirates and Mayors had lodged a complaint stating that the Czech Statistical Bureau misinterpreted the electoral law, resulting in an incorrect allocation of seats.

[272] Another complaint that received media attention was made by Zdeněk Černý, who was due to run as a candidate for SPOLU in South Moravia before his candidacy was withdrawn in the last moments before the election.

Černý argued his withdrawal was illegal and wanted to invalidate the election of all SPOLU candidates in South Moravia to recount the preferential votes.

SPD leader Tomio Okamura said in a press conference that passing a new law to allow national referendums, including on Czexit and possibly NATO membership, was his condition for supporting Babiš' campaign for a second term in office.

After results showed that a potential ANO–SPD majority was mathematically impossible, Babiš indicated his willingness to form a grand coalition with SPOLU, if he were to be asked first by Zeman.

[290] In response, the Senate announced their intention to transfer Zeman's constitutional powers to the current prime minister, Andrej Babiš, and the speaker of the Chamber of Deputies.

[292] Ministries held by Spolu include Finance, Labour and Social affairs, Defence, Transportation, Health, Agriculture, Justice, Environment, Culture and Science.

[338] The vote was also delayed by Freedom and Direct Democracy leader Tomio Okamura, who called for a special meeting over the financing of Mayors and Independents, following media reports that the party had accepted gifts from Cyprus.