Liga Deportiva Universitaria de Quito's 2021 season was the club's 91st year of existence, the 68th year in professional football, and the 60th in the top level of professional football in Ecuador.

Supplier: Puma Sponsor(s): Banco Pichincha, Mazda, CNT, Discover, Pilsener, Salud SA

Note: Caps and goals are of the national league and are current as of the beginning of the season.

Quito qualified to the 2021 CONMEBOL Libertadores—their 19th participation in the continental tournament—as Runner-up of the 2020 LigaPro.

Source:[13] Note: Players in italics left the club mid-season.