The six-team single-elimination tournament consisted of three rounds based on seeding from regular season conference play.
[1][2] The conference tournament title was the fourth for the Loyloa women's soccer program, all of which have come under head coach Barry Bimbi.
Six of the nine Missouri Valley Conference women's soccer programs qualified for the 2021 Tournament.
Drake, Evansville, and Illinois State all finished with identical 2–3–3 records in the regular season.
Illinois State did not qualify for the tournament after earning .5 points per match against the top four seeds.