The 2021 NCAA Division II women's basketball tournament was the 39th annual tournament hosted by the NCAA to determine the national champion of Division II women's collegiate basketball in the United States.

[1] Defending champions Lubbock Christian defeated Drury in the championship game, 69–59, to claim the Lady Chaps' third overall and second consecutive national title.

The field size was temporarily reduced for just the 2021 championship to account for teams and conferences that chose to not compete during the 2020–21 season due to the COVID-19 pandemic.

Teams from six conferences (CCAA, CIAA, Northeast-10, PSAC, SIAC and Sunshine State) did not participate in the regular season and were ineligible for tournament bids.

As a result, each regions consists of two to three automatic qualifiers (the teams who won their respective conference tournaments) and two to four at-large bids.