[6] On April 21, Orlando Pride beat Washington Spirit 1–0 in the Challenge Cup, their first win since August 21, 2019, snapping a streak of 13 winless games in 609 days.

[7] Four Orlando Pride players were selected to represent their nations at the delayed 2020 Tokyo Olympics in July: Marta, Erin McLeod, Alex Morgan and Ali Riley.

[13] On July 28, it was announced Alex Leitão, the chief executive officer of the Orlando City SC organization since 2015, was stepping down from his role.

[14] On October 1, the NWSL announced all scheduled games would not take place that weekend following allegations of historic abuse and sexual coercion against former North Carolina Courage coach Paul Riley.

[15] Riley was the fourth head coach to be sacked having been accused of misconduct during the 2021 season after Farid Benstiti, Richie Burke and Christy Holly.

Orlando Pride were placed in the East division with North Carolina Courage, NJ/NY Gotham FC, Washington Spirit, and new expansion franchise Racing Louisville.

[28] Mikayla Colohan and Kerry Abello both announced they would return to their respective colleges for the 2021 season and exhaust their final year of eligibility.

[26] They were later joined by Gabby English who had most recently been in Portugal with Boavista and Jamaican international Lauren Silver who had spent the 2020 Fall Series signed to Houston Dash.