Diede de Groot defeated Kgothatso Montjane in the final, 6–2, 6–2 to win the ladies' singles wheelchair tennis title at the 2021 Wimbledon Championships.

[1] It was her third Wimbledon singles title, completed a non-calendar-year Grand Slam, and was the third step in an eventual Super Slam.

Aniek van Koot was the defending champion from when the event was last held in 2019,[2] but was defeated in the quarterfinals by Jordanne Whiley.