The 49ers played their home games at Jerry Richardson Stadium in Charlotte, North Carolina, and competed in Conference USA (C–USA).

Charlotte fired Healy after eight games; offensive line coach Peter Rossomando finished out the season.

[2] On February 16 Healy announced the hiring of Greg Brown as co-defensive coordinator and Kap Dede as secondary coach.

Rossomando finished out the season as interim head coach with a Senior Day win over Louisiana Tech.

• OL – Ashton Gist, Sr. Defense: DE – Markees Watts, R-Sr. • CB – Geo Howard, R-Sr. Special Teams: KR – Shadrick Byrd, R-So.