Florida competed in the Eastern Division of the Southeastern Conference (SEC).

Home games were played at Condron Ballpark[note 1] on the university's Gainesville, Florida, campus, in the second season at the ballpark.

The Gators entered the season looking to return to the College World Series after an early regional exit from the 2021 NCAA tournament.

1 team, but stumbled to a 17–13 SEC record to enter the SEC tournament as the sixth seed, where they made the semifinals before being eliminated by eventual tournament runner-up Tennessee.

The Gators were then selected to host a regional in the 2021 NCAA Division I baseball tournament in the Austin Super Regional, but were eliminated in only two games, suffering a 19–1 loss to South Alabama, who scored 10 runs with two outs in the sixth inning.