During the 2023–24 season, Jong Ajax participated in the Dutch Eerste Divisie, the 2nd tier of professional football in the Netherlands.

It is their 10th consecutive season in the Eerste Divisie.

Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.

For a list of all Dutch football transfers in the summer window (13 June 2023 to 1 September 2023) please see List of Dutch football transfers summer 2023.

For a list of all Dutch football transfers in the winter window (2 January 2024 to 1 February 2024) please see List of Dutch football transfers winter 2023–24.