The season began on 20 August 2023 and ended on 26 May 2024.
Schifflange 95 and Marisca Mersch were promoted to the Luxembourg National Division at the end of the previous season.
They were replaced by Hostert, who finished in the bottom two positions of the 2022–23 Luxembourg National Division and Etzella Ettelbruck.
FC Atert Bissen, Schieren, Luxembourg City and Junglinster were relegated at the end of the previous season and were replaced by FC Koeppchen Wormeldange, FC Lorentzweiler, Walferdange and FC Avenir Beggen, who earned promotion from the Luxembourg 1.
The thirteenth and fourteenth-placed teams (Lorentzweiler and Weiler) each faced one of the two runners-up from the Luxembourg 1.