The 2023 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts.

It was the 19th edition of the tournament which was part of the 2023 ATP Challenger Tour.

It took place in Guayaquil, Ecuador between 30 October and 5 November 2023.

The following players received wildcards into the singles main draw: The following players received entry from the qualifying draw: The following player received entry as a lucky loser: