The 2023 Knoxville Challenger was a professional tennis tournament played on indoor hard courts.

It was the 19th edition of the tournament which was part of the 2023 ATP Challenger Tour.

It took place in Knoxville, United States between 6 and 12 November 2023.

The following players received wildcards into the singles main draw: The following player received entry into the singles main draw as a special exempt: The following players received entry from the qualifying draw: The following players received entry as lucky losers: