In the league includes the MFF's youth team called Junior Lions and nine other clubs.
If University does not pass the MNL criteria, Port FC is promoted to 2024 Myanmar National League.
Mawyawadi FC and Golden Grit relegated to Myanmar Amateur League.
Junior Lions is a team consisting of players under the age of 18, and as a club based in MFF, they are also unable to be promoted.
Newly founded clubs were Glory Goal, Shwe Pyi Thar, Port, Golden Grit and Thitsar Arman FC.