The 2024–25 LEN Women's Champions League is the 37th edition of the water polo competition organised by European Aquatics.

German representative, Spandau 04 and Israeli team, Hapoel Yoneam were the only clubs outside the top 6 who decided to enter, starting in the qualifiers.

All other clubs and countries below the top six entered the 2024–25 LEN Women's Challenger Cup.

Dunaújváros Antenore Plebiscito Padova (H) NO Vouliagmeni SIS Roma FTC Telekom CN Terrassa Ethnikos Piraeus ANC Glyfada CN Sant Andreu Lille UC Digi Eger (H) Alimos NAC Betsson ZV De Zaan BVSC-Zugló Spandau 04 (H) Pallanuoto Trieste Grand Nancy AC EPlus CN Catalunya Tenerife Echeyde Panionios GSS (H) III.

Keruleti TVE Rapallo Pallanuoto Polar Bears (H) Widex GZC Donk Hapoel Yoneam