Sydney FC were the defending champions, but were eliminated by Oakleigh Cannons in the Round of 32.
Macarthur FC won its second Australia Cup after defeating Melbourne Victory in the final.
For the first time in the competition's history, the draws for the round of 16, quarter-finals and semi-finals are combined.
All times listed below are at AEST The lowest ranked sides that qualified for this round was South Melbourne.
All times listed below are at AEST Note: Goals scored in preliminary rounds not included.