The 2024 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts.

It was the 20th edition of the tournament which was part of the 2024 ATP Challenger Tour.

It took place in Guayaquil, Ecuador between 28 October and 3 November 2024.

The following players received wildcards into the singles main draw: The following player received entry into the singles main draw using a protected ranking: The following players received entry into the singles main draw as alternates: The following players received entry from the qualifying draw: