Unlike previous editions, this edition did not automatically grant berths based on the national club ranking; instead, of the 92 available berths, 80 were distributed between the state leagues, with the remaining 12 being allocated to the teams qualified for the Copa Libertadores, the champions of regional cups Copa Verde and Copa do Nordeste, the champion of the second division Série B, and any remaining berths going to non-qualified clubs in Série A.
As champions, Flamengo qualified for the 2025 Copa Libertadores group stage and the 2025 Supercopa do Brasil.
The 32 teams, qualified from the previous round, were placed into two pots, seeded by their national ranking, and then drawn into 16 ties.
If the aggregate score was level, the second-leg match would go straight to a penalty shoot-out to determine the winners.
[12] Owing to requests from Flamengo and Atlético Mineiro due to the FIFA Calendar, the dates of the second leg matches were changed from 17 to 19 and 20 October.