The top two teams from each group advanced to the knockout stage to compete in a single-elimination tournament.
There were 32 matches in the knockout stage, including a third-place playoff played between the two losing semifinalists.
Matches in the knockout stage were played to a finish.
The top two placed teams from each of the top fifteen groups qualified for the knockout stage, joining América and Columbus Crew, who both received byes to this round.
The winner of the third place match qualified for the first round of the 2025 CONCACAF Champions Cup.