The 2024 Lexington Challenger was a professional tennis tournament played on outdoor hard courts.

It was the twenty-ninth edition of the tournament for men, which was part of the 2024 ATP Challenger Tour, and the twenty-seventh edition for women, which was part of the 2024 ITF Women's World Tennis Tour.

It took place in Lexington, Kentucky, United States, between 29 July and 4 August 2024.

[1] The following players received a wildcard into the singles main draw: The following player received entry into the singles main draw as a special exempt: The following player received entry into the singles main draw as an alternate: The following players received entry from the qualifying draw: The following players received entry as lucky losers: The following players received wildcards into the singles main draw: The following player received entry into the singles main draw using a special ranking: The following player received entry into the singles main draw as a special exempt: The following player received entry into the singles main draw through the College Accelerator Programme: The following players received entry from the qualifying draw: