The 2024 Macha Lake Open was a professional tennis tournament that was played on outdoor clay courts.

It was the twenty-first leg of the tournament, which was part of the 2024 ITF Women's World Tennis Tour.

It took place in Staré Splavy, Czech Republic, between 24 and 30 June 2024.

[1] The following players received wildcards into the singles main draw: The following player received entry into the singles main draw using a special ranking: The following player received entry into the singles main draw through a special exempt: The following players received entry from the qualifying draw: