[1] The USL Regular season was historically bad as Louisville finished fifth in the Eastern Conference; the lowest in team history.

[2] In the USL Playoff Louisville returned to form as they reached the Eastern Conference finals for the ninth consecutive season.

This included long time captain Paolo DelPiccolo who retired from on field play to take a coaching position with the team and all-time leading goal scorer Cameron Lancaster signing with Lexington SC.

[6] The preeminent signing was Arturo Ordoñez, the reigning USL Defensive Player of the year, from rival Pittsburgh.

[11] The most significant event during these matches was an injury to team captain Brian Ownby who injured his leg in the final friendly against Knoxville.

[12] Louisville kicked off the USL regular season away from home against Western Conference Side El Paso in a match were seven players made their debuts for the team.

The match remained scoreless until the second half when Wilson Harris scored the games only goal after a Sean Totsch cross.

Four different players found the back of the net as Taylor Davila and Jake Morris scored their first goals in a Louisville jersey while Harris registered a brace.

Indy would then level the score on a penalty kick by Jack Blake only for Wilson to put Louisville back in front before the half time whistle.

[20] Three days later Louisville returned to the road to face Charleston in a rematch of the previous year's Eastern Conference final in front of a soldout crowd.

Approximately 10 minutes later Kyle Adams was called for a handball in box giving a Charleston penalty kick.

On the defensive end of the pitch Danny Faundez made his first start of the year in goal earning a clean sheet.

Of those six players Adrien Perez, Aiden McFadden, and Tola Showunmi scored their first goals in Louisville Jerseys while Jake Morris contributed two assists.

[24] Louisville's largest margin of victory in a league game since defeating New York Red Bulls II 6–0 in July 2022.

[26] Louisville began May at home against Western Conference side Orange County SC on short rest after playing in the U.S. Open Cup midweek.

After the half, Danny Cruz made several substitutions including bringing on Serrano who drew Louisville level in the 70th minute.

[30] Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.

Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.

As a member of the USL Championship that finished outside the top eight teams in the 2023 season, Louisville City entered the tournament in the Third Round, with their Cup opener played on April 16, 2024.