The 2024 NCAA Division I softball season, play of college softball in the United States organized by the National Collegiate Athletic Association (NCAA) at the Division I level, began in February 2024.

The Women's College World Series, consisting of the eight remaining teams in the NCAA Tournament and held annually in Oklahoma City at Devon Park, ended in June 2024.

Two softball-sponsoring schools started transitions from D-II after the 2024 season: In addition to these changes, the Indiana University and Purdue University systems split IUPUI into separate IU- and Purdue-affiliated institutions at the end of the 2023–24 academic year, a move similar to the two systems' dissolution of their joint Fort Wayne campus in 2018.

The teams in each conference that win their regular season title are given the number one seed in each tournament.

Conference tournament winners plus the Big West and West Coast Conference regular-season champions receive automatic invitations to the 2024 NCAA Division I softball tournament.