SC MesoAmerica (CA) and NTX Image (TX) were the defending Men's and Women's National Amateur Cup champions, respectively.

In total, 16 teams across seven state associations[a] in USASA Region I and the National Premier Soccer League sent representatives to the tournament for the Fitz Marth Amateur Cup.

Bracket Home teams listed on top of bracket[b] Bold = winner * = after extra time, ( ) = penalty shootout score, FF = forfeit In total, 4 teams across four state associations participated in a round-robin tournament on June 15 and 16 at the Ukrainian American Sports Center in North Wales, Pennsylvania alongside the men's regional final.

In total, 24 teams across seven state associations in USASA Region II and the National Premier Soccer League sent representatives to the tournament for the Bill Davey Amateur Cup.

In total, four teams from two state associations participated in a single-elimination knockout tournament across late June and early July to determine Region II's women's representative.

The bracket included 2021 National Amateur Cup champions Rockford Raptors and 2024 Finals host DeKalb County United.

The final was played at Montrose Turf Field in Chicago on July 2.Bold = winner * = after extra time, ( ) = penalty shootout score, FF = forfeitIn total, 8 teams across 12 state associations in USASA Region III and the National Premier Soccer League sent representatives to the tournament.

[a] Bracket Home teams listed on top of bracket[b] Bold = winner * = after extra time, ( ) = penalty shootout score, FF = forfeit The Region IV women's representative was intended to be decided via a four-team tournament featuring teams from four state associations (California, Nevada, New Mexico, and Utah) in a Las Vegas-hosted single-elimination tournament.

However, scheduling issues led to the cancelation of the event and an agreement that Pan World Elite WFC (Utah) would serve as the region's representative in the national finals.