The Blue Jays gained a compensation selection at the end of the fourth round due to Matt Chapman signing with the San Francisco Giants after having been given a qualifying offer.
[12] As of August 2024[update], four players on the Opening Day roster are no longer with the team: Cavan Biggio, Isiah Kiner-Falefa, Kevin Kiermaier and Justin Turner.
Of the starters, the only players that remain are George Springer, Daulton Varsho, Vladimir Guerrero Jr., Alejandro Kirk and José Berríos.
[27] Heading to the newly renovated Rogers Centre for the first time in 2024, the Blue Jays defeated the Seattle Mariners 5–2 in their home opener, and would take two of three games to win their first series of the season.
Newly signed pitcher Yariel Rodríguez made his debut during the series where he struck out six batters and only got one earned run but did not get credited with the win.
[32] The Blue Jays then continued to Kansas City to take on the Royals, where they lost three of the four games, all by one run; the last was called after the top of the fifth due to rain.
The first game was marked by two-way player Shohei Ohtani hitting a home run in the first inning after he was booed by Toronto fans, following an off-season in which he was reported by some journalists to be signing with the team.
[38] They then lost a series against the Twins at home,[39] followed up by a two-game (middle game was rained out and was made up in a doubleheader later in the season) split against the Orioles at Camden Yards.
[41][42] From May 23 to 26, the Blue Jays lost three of four to the Detroit Tigers on the road, punctuated by a 14–11 loss in the final game after Matt Vierling hit a three-run walk-off home run.
[45] On May 31, the Blue Jays debuted their new City Connect Jerseys with the theme entitled "Night Mode" where they played against the Pittsburgh Pirates where they won at the bottom of the 14th inning thanks to a walk-off home run from Davis Schneider.
[46] On June 23, infielder Orelvis Martínez, who made his MLB debut a few days earlier, was suspended 80 games for testing positive for performance-enhancing substance clomifene, which he claims is being taken for addressing fertility issues.