Before the war, most of the regiment's men were members of "The Old Infantry" a state guard unit under the command of Captain Samuel Woodson Price, who would later command the regiment.
The regiment was attached to 11th Brigade, 1st Division, Army of the Ohio, to March 1862.
23rd Brigade, 5th Division, II Corps, Army of the Ohio, to November 1862.
3rd Brigade, 3rd Division, Left Wing, XIV Corps, Army of the Cumberland, to January 1863.
3rd Brigade, 3rd Division, XXI Corps, Army of the Cumberland, to October 1863.