The 21st Virginia Infantry Regiment was an infantry regiment raised in Virginia for service in the Confederate States Army during the American Civil War.

Company B was known as the Maryland Guard and recruited among Southern sympathizers in the border state.

After participating in Lee's Cheat Mountain and Jackson's Valley campaigns, the unit was assigned to J.R. Jones's and W.Terry's Brigade, Army of Northern Virginia.

It took an active part in many conflicts from the Seven Days' Battles to Cold Harbor, then fought with Early in the Shenandoah Valley and the Appomattox Campaign.

This article about a specific military unit of the American Civil War is a stub.