The 25th Kentucky Infantry Regiment was organized at Camp Joe Anderson near Hopkinsville, Kentucky and mustered in for a three-year enlistment on January 1, 1862.
The regiment was attached to 13th Brigade, Army of the Ohio, to December 1861.
Moved to Fort Donelson, Tennessee, February 11–13.
Investment and capture of Fort Donelson, February 13–16.
The regiment lost so many men to disease, that it was consolidated with the 17th Kentucky Infantry.