The 25th Virginia Cavalry Regiment was a cavalry regiment raised in Virginia for service in the Confederate States Army during the American Civil War.

It fought mostly in western Virginia and the Shenandoah Valley.

Serving in McCausland's and Imboden's Brigade, the unit fought in numerous engagements in western Virginia and the Shenandoah Valley.

Its commanders were Colonel Warren M. Hopkins, Lieutenant Colonel Henry A. Edmundson, and Major Sylvester R. McConnell.

This article about a specific military unit of the American Civil War is a stub.