The 27th Kentucky Infantry Regiment was organized at Rochester, Kentucky and mustered in for a three-year enlistment on March 21, 1862, under the command of Colonel Charles D. Pennebaker.
The regiment was attached to 19th Brigade, 4th Division, Army of the Ohio, to September 1862.
19th Brigade, 4th Division, II Corps, Army of the Ohio, to November 1862.
2nd Brigade, 2nd Division, Left Wing, XIV Corps, Army of the Cumberland, November 1862.
District of Western Kentucky, Department of the Ohio, to June 1863.
Unattached, 2nd Division, XXIII Corps, Army of the Ohio, to August 1863.
Unattached, Munfordville, Kentucky, 1st Division, XXIII Corps, to October 1863.
3rd Brigade, 1st Division, Cavalry Corps, Department of the Ohio, to April 1864.
The regiment was at Elizabethtown and Grayson Springs, Kentucky, until March 1862.
Buell's Campaign in northern Alabama and middle Tennessee June to August.
The regiment did post duty at Munfordville as well as guarding the line of the Louisville & Nashville Railroad until September, 1863.
Joined Manson at Glasgow, Ky., and march to Knoxville, Tenn., September 1863.
Battles about Dallas, New Hope Church and Allatoona Hills May 25-June 5.
Duty at Louisville and at Owensboro, Ky., operating against guerrillas until March 1865.