The 27th Battalion Virginia Partisan Rangers was a cavalry regiment raised in Virginia for service in the Confederate States Army during the American Civil War.

Virginia's 27th Battalion Partisan Rangers was formed in September, 1862, with seven companies, later increased to nine.

Jones' Brigade and participated in various engagements in East Tennessee and western Virginia.

During April, 1864, it contained 240 effectives, and in July it merged into the 25th Virginia Cavalry Regiment.

This article about a specific military unit of the American Civil War is a stub.