The 28th Iowa Infantry Regiment was an infantry regiment that served in the Union Army during the American Civil War.

Iowa circuit court judge William E. Miller retired from the bench to become the colonel of the regiment at Camp Pope, near Iowa City.

For two months they engaged in drilling of recruits, and in November of that year marched through Missouri to an encampment at Helena, Arkansas.

The regiment engaged in various expeditions, but Miller "contracted a disease which meant death in the South", which forced him to return to Iowa in March 1863.

This article about a specific military unit of the American Civil War is a stub.