The regiment is part of the army's infantry corps' Bersaglieri speciality and was last operationally assigned to the Armored Brigade "Centauro".

On 31 December 1861 the command was renamed 2nd Bersaglieri Regiment, but continued to exert only administrative functions.

In 1866, in preparation for the Third Italian War of Independence, the regiment formed the XLII Battalion, which was disbanded in December 1870.

On 6 May 1848, during the Battle of Santa Lucia, the II Battalion's 4th Company distinguished itself fighting inside the walls of the city of Verona.

The two provisional battalions were part of the Sardinian Expeditionary Corps, which fought in the Crimean War.

In 1859, during the Second Italian War of Independence, II and IV battalions fought in the Battle of Solferino.

On 15 April 1860 the Royal Sardinian Army formed the XVII Battalion with volunteers, who had fought in the Second Italian War of Independence.

In 1895-96 the regiment provided 13 officers and 278 troops to help form the I, III, V, and VI provisional battalions, which were deployed to Eritrea for the First Italo-Ethiopian War.

In 1911, the regiment provided 22 officers and 899 troops to augment units fighting in the Italo-Turkish War.

In January 1915 the depot of the 2nd Bersaglieri Regiment in Rome formed the LIII and LIV battalions.

[4][6] On 24 May 1915, the day after Italy's entry into the war, the regiment occupied the Cereda Pass in the Dolomites.

In August and September 1915 the regiment repeatedly attacked Austro-Hungarian positions on Monte Coston.

[9] The same year the depot also formed the XXVI Assault Unit, which was part of the Arditi shock troops.

On 25 October the regiment began to retreat towards along the Isonzo river, but found all the bridges had been destroyed.

The remainder of the regiment, two and a half battalions strong, was forced to surrender to the Austro-Hungarian troops.

On 30 October the VII brigade attacked towards Oderzo and from there rapidly advanced to San Vito al Tagliamento in pursuit of the fleeing Austro-Hungarian armies.

In May 1917, during the Tenth Battle of the Isonzo the battalion fought on the Monte Santo di Gorizia.

[4][6] After the Battle of Caporetto the battalion fought a delaying action at the bridge over the Torre river at Nimis, and then at San Daniele del Friuli.

On 5 November the battalion fought against advancing enemy forces at the bridge over the Meduna river at Navarons.

For its service at Oslavia, at Zaibena, on Nad Logem, and on the Monte Santo the II Cyclists Battalion was awarded a Silver Medal of Military Valor, which was affixed to the flag of the 2nd Bersaglieri Regiment and added to the regiment's coat of arms.

On 11 March 1932 the regiment formed a motorized unit, which consisted of four platoons of Bersaglieri on motorcycles.

On 8 September 1943, the day the Armistice of Cassibile was announced, the regimental command and XVII Battalion were in Chalcis and quickly overcome by invading German forces.

[12] On 12 November 1976 the President of the Italian Republic Giovanni Leone assigned with decree 846 the flag and traditions of the 2nd Bersaglieri Regiment to the battalion.