The regiment was attached to Dix's Division, Army of the Potomac, to March 1862.

Eastern Shore Maryland and Virginia Middle Department, to July 1862.

District of Eastern Shore, VIII Corps, Middle Department, to March 1863.

2nd Brigade, 1st Division, XII Corps, Army of the Potomac, July 1863.

2nd Brigade, Maryland Heights, Division of West Virginia, to December 1863.

Reserve Division, District of Harpers Ferry, West Virginia, to February 1865.

The 2nd Maryland Infantry, Eastern Shore ceased to exist on February 23, 1865 when it was consolidated with the 1st Regiment Eastern Shore Maryland Volunteer Infantry.

Duty on the eastern shore of Maryland until March 1862, and at Baltimore until October 1862.