3,5-Difluoro-4-hydroxybenzylidene imidazolinone or DFHBI[2] is an imidazolinone fluorophore used in various biochemical studies.

The benzene ring of DFHBI can freely rotate around the single bond but when it is fixed in planar conformation, DFHBI fluoresces.

[1] It is a synthetic analog of the GFP fluorophore.

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