The regiment was attached to District of Western Kentucky, Department of the Ohio, to April 1863.
2nd Brigade, District of Western Kentucky, Department of the Ohio, to June 1863.
1st Brigade, 1st Division, XXIII Corps, Army of the Ohio, to August 1863.
The 32nd Kentucky Infantry mustered out of service beginning on May 28, 1863, and ending on August 12, 1863.
Engaged in guard and scouting duty at various points in District of Western Kentucky, principally at Hopkinsville, Camp Burnside, Danville, Lexington, Somerset, Stanford, and Lebanon.