Although it is administered from Queen Elizabeth Barracks in North Yorkshire, as a specialist unit the regiment recruits reservists from all over the UK.

335 Medical Evacuation Regiment was formed on 1 April 2005 from the Ambulance Train Group (Volunteers).

The Regiment under the integrated review trains high and medium dependency care clinicians and general ambulance crews to deploy on operations and exercises around the world either as an individual augmentee or as part of a formed unit.

Each Squadron comprises several Troops which specialise in high, medium dependancy and general crew capabilities.

The regiment is under 2 Medical Group, Field Army Troops and supports units as tasked.