The regiment as a whole saw action with the Army of the Tennessee at Fort Donelson, Shiloh, Corinth, Hatchie's Bridge, Vicksburg and Meridian.

This non-veteran detachment fought at Fort DeRussy, Pleasant Hill, Mansura and Tupelo.

They were assigned to guard duty along the railroad near Big Shanty, Marietta and Kenesaw Mountain.

Then moved with the army during the March to the Sea and siege of Savannah, Georgia.

The regiment suffered 8 officers and 107 enlisted men who were killed in action or mortally wounded and 3 officers and 107 enlisted men who died of disease, for a total of 225 fatalities.