The 48th Wisconsin Infantry Regiment was a volunteer infantry regiment that served in the Union Army during the American Civil War.

It was organized under the supervision of Lieutenant Colonel Henry B. Shears, Colonel Uri B. Pearsall being at the time in service as lieutenant colonel of a colored regiment.

The regiment was mustered out on March 24, 1866, at Fort Leavenworth, Kansas.

The 48th Wisconsin suffered 16 enlisted men who died of disease, for a total of 16 fatalities.

This article about a specific military unit of the American Civil War is a stub.