All were mustered in for one year under the command of Colonel John H. Grider.
The regiment was attached to 3rd Brigade, 1st Division, District of Kentucky, Department of the Ohio, to July 1864.
2nd Brigade, 2nd Division, District of Kentucky, Department of the Ohio, to October 1864.
The 52nd Kentucky Mounted Infantry mustered out of service January 17, 1865.
Operations against guerrillas in southern and central Kentucky, guarding and protecting public property, and protecting lines of communication with the army operating at the front until March 1864.