The 53rd Illinois General Assembly met from 1923 to 1925.

Fred E. Sterling of Rockford was the Lieutenant Governor of Illinois and thus ex officio President of the Senate.

[a] Richard J. Barr of Joliet was President pro tempore of the Senate.

David Shanahan of Chicago was the Speaker of the House of Representatives.

Illinois was divided into 51 districts, each of which elected one Senator and three Representatives.