After training in the United States, it deployed to the European Theater of Operations, where it participated in the strategic bombing campaign against Germany.

The squadron researches, builds, ships, tests, and maintains software applications to support mission essential functions.

These functions include rapid electronic warfare integrated reprogramming, electromagnetic spectrum operations assessment, and target and waveform capability development.

Its Detachment 1 serves as a software development training arm to enable others by prioritizing a data-first, test driven approach to secure electromagnetic spectrum operations superiority.

[4][5] The cadre that formed at Gowen moved to Wendover Field, Utah in February 1943, where the unit was fully manned and squadron training with Boeing B-17 Flying Fortress heavy bombers began.

The squadron primarily engaged in the strategic bombing campaign against Germany, attacking industrial sites, oil refineries and storage facilities, communications centers and naval targets on the European Continent.

[5] The squadron was awarded a Distinguished Unit Citation (DUC) for attacking an aircraft factory at Regensburg, Germany, on 17 August 1943, withstanding heavy resistance to reach the target.

[5] Other strategic targets included aircraft factories at Brunswick, Kassel, and Reims; airfields at Paris, Berlin and in Bordeaux; naval installations at Emden, Kiel and La Pallice, chemical works in Ludwigshafen; ball bearing factories at Schweinfurt and rail marshalling yards in Bielefeld, Brussels, and Osnabruck.

It attacked military installations in France in early 1944 to help prepare the way for Operation Overlord, the invasion of Normandy, and on D Day hit coastal defenses, artillery batteries and transportation targets.

It struck military installations and airfields near Arnhem during Operation Market Garden, the unsuccessful attempt to secure a bridgehead across the Rhine in the Netherlands.

[4][8] Although nominally a bombardment unit, the squadron was equipped with North American AT-6 Texan and Beechcraft AT-11 Kansan trainers.

President Truman's reduced 1949 defense budget also required reductions in the number of units in the Air Force,[11] At O'Hare, the 338th Group and its squadrons were inactivated, and most of its personnel transferred to the 437th Troop Carrier Wing.

It deployed to Wheelus Air Base, Libya in April 1955, where it was able to train in gunnery and bombing for the first time since arriving in Europe.

Starting in November 1955, it began deployments to Detachment 1 of the 388th Wing at Hahn Air Base, Germany to stand nuclear alert.

In April 1965, the 563d deployed to Takhli Royal Thai Air Force Base, Thailand, where it participated in combat operations in the Vietnam War under the control of the 6235th Tactical Fighter Wing[h][7][22] On 27 July 1965, the unit participated in the first destruction of a surface-to-air missile site in North Vietnam in Operation Iron Hand.

It participated in the first increment of Operation Rolling Thunder, and in several attempts to destroy the Thanh Hoa and Paul Doumer Bridges.

In October 1978, the 563rd received new aircraft from Nellis Air Force Base, Nevada, and was the first operational squadron to fly the advanced F-4G Wild Weasel.