In 1990 when the Radiological, Chemical, and Biological Protection Troops within the Armed Forces of Ukraine were established, they numbered five brigades and 15 similar battalions of this type.

In July 2007, the regiment was fulfilling the task on eliminating the consequences of the accident at the Ozhidiv-Krasne railway line of the Lviv Railway (uk:Фосфорна аварія під Ожидовом), where 15 tanks with yellow phosphorus were upturned.

The military property (machines, garages, hangars) was sold, and personnel dismissed or transferred to other units.

In December 2013, the last 20 CRM-4 (Chemical Reconnaissance Machine) vehicles based on armored personnel carrier (BTR-80) were sent to Crimea.

[1] With the beginning of the Russo-Ukrainian War (c2014), regiment personnel with machine guns and sapper shovels in their hands took part in the protection of the territorial integrity of Ukraine.