Following the opening of the entire Kyushu Shinkansen route on 12 March 2011, 800 series trains were primarily used on all-stations Tsubame services operating mostly between Hakata and Kumamoto.

Set U005 was withdrawn on 16 March 2018 following damage sustained from the 2016 Kumamoto earthquakes' 14 April foreshock.

More traditional materials and design features are used to embody Kyushu's warm and natural environment.

Persimmon tannin colour is used for the walls, ancient lacquer for the doors, and Kyushu traditional rope curtain from Yatsushiroigusa for the lavatory are used.

The seats are made of wood versus metal/plastic combinations from previous trains and the cushions are produced using Nishijin textiles.