The 89th Infantry Regiment was constituted in the Regular Army on 31 August 1918 and organized in September 1918 at Camp Travis, Texas, as an element of the 20th Division.

However, World War I ended before the unit could deploy overseas, and the 89th Infantry was demobilized from 27-29 March 1919 at Fort Oglethorpe, Georgia, and Camp Sevier, South Carolina.

The elements of the regiment guarded various U.S. installations in Bermuda and the Caribbean before being inactivated beginning in 1943.

The HHC, 3rd Battalion and Companies I, K, and M were inactivated on 21 October 1943 at Fort Benning, Georgia.

The regiment as awarded the American Theater campaign streamer without inscription for its service.