It mustered in under the command of Colonel James Murrell Shackelford.
The regiment was attached to District of Louisville, Kentucky, Department of the Ohio, to November 1862.
It was unattached at Bowling Green, District of Western Kentucky, Department of the Ohio, to June 1863; attached to the 1st Brigade, 2nd Division, XXIII Corps, Army of the Ohio, to August 1863; unassigned, at Bowling Green, Kentucky; and attached to the 1st Division, XXIII Corps, to September 1863.
Duty at Russellville, Bowling Green and Hopkinsville, Kentucky, District of West Kentucky, and at Clarksville, Tennessee, operating against guerrillas, until September 1863.
Expedition from Bowling Green, Kentucky, to Tennessee State Line May 2–6.